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1. To develop a thorough understanding and knowledge of aseptic technique and culture transfer procedures.
2. To study on how to maintain the sterility during microbiological work.
3. To learn how to safety transfer cultures of microorganisms from one container to another without contaminating the cultures or exposing yourself to them.
4. To demonstrate skill in isolation of microorganisms from a mixed culture using selective and differential media.
5. To isolate microorganisms from a wide variety of sources and describe their colonial morphology.
6. TO LEARN HOW TO TRANSFER A CULTURE FROM ONE VESSEL TO ONE ANOTHER.

Based on the above reasons, it can be concluded that the objective is Successfully achieved. There is no growth of any microorganism in the nutrient agar or broth when using the aseptic technique. Without aseptic technique growth of microorganisms on the nutrient agar is observed but not in nutrient broth which is clear as initial condition. By the end of this experiment, students learnt on how to perform microbiological work by maintaining the laboratory condition, methods and techniques which will render sterility called aseptic technique.